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Raybio G-series

RayBio® G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

RayBio® G-Series Human Apoptosis Antibody Array

Antibody arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid.

With the help of Antibody Arrays, researchers are also gaining a better understanding of cancer cells, mutations and antibodies. The RayBio® Human Apoptosis Antibody Array can detect 43 different apoptotic proteins (including p53) with picogram-per-milliliter (pg/ml) sensitivity. Researchers studying the deregulation of apoptosis in diseases such as coronary artery disease, HIV infection and cancer can use the array to increase detection of apopotic proteins within their samples.

The kit consists of the arrays and all reagents necessary for detecting these proteins in cell and tissue lysates.

The RayBio® Human Apoptosis Antibody Array utilizes a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence. 

Do not have a fluorescent microarray scanner in your lab? No worries! Genomax provides Scan and Report service for customers working with the glass slide format (G-series) antibody array. The signal intensities obtained from microarray laser scanner can simply be imported into our Data Analysis Tool.

Please contact Genomax for the walk-through of the technology. 

Array Map

Human Apoptosis Array G1 (43 apoptotic proteins)

bad
bax
bcl-2
bcl-w
BID
BIM
Caspase-3
Caspase-8
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
HSP70
HTRA2
IGF-1
IGF-2
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1 R
livin
p21
p27
p53
SMAC
Survivin
(BIRC5)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
XIAP

 

Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

How It Works

G-Series Antibody Arrays utilize the sandwich-ELISA design principle. In this assay, capture antibodies are printed in multiple identical arrays on a standard-sized histology slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are visualized using a fluorescence laser scanner.

Publications cited RayBio Apoptosis Antibody Arrays

    1. Asif M., Al-Masoub M., Sultan Kahn M., et al. Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells. Journal of Applied Biomedicine, Oct 18 2016, epub ahead of print
      Species: Human
      Sample Type: Cell Lysate

    2. Asif M., Shafaei A., Jafari S., et al. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicol Lett. 2016 Aug 22;257:84-96. doi: 10.1016/j.toxlet.2016.05.027.
      Species: Human
      Sample Type: Cell Lysate

    3. Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325.

    4. Mohidin T., Ng C. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells. J Biosci. 2015 Mar;40(1):41-51.
      Species: Human
      Sample Type: Cell Lysate

    5. Qin M., Luo Y., Meng X., et al. Myricitrin attenuates endothelial cell apoptosis to prevent atherosclerosis: An insight into PI3K/Akt activation and STAT3 signaling pathways. Vascul Pharmacol. 2015 Jul;70:23-34. doi: 10.1016/j.vph.2015.03.002
      Species: Human
      Sample Type: Cell Lysate

    6. Ibrahim M., et al. α-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-κ and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27;8:1629-47. doi: 10.2147/DDDT.S66105
      Species: Human
      Sample Type: Cell Lysate

    7. Li X., Liu X., Xu Y., et al. Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins. Int J Oncol. 2015 Feb;46(2):677-84. doi: 10.3892/ijo.2014.2780.
      Species: Human
      Sample Type: Cell Lysate

    8. Sidhu P., et al. Anticancer activity of VDR-coregulator inhibitor PS121912. Cancer Chemotherapy and Pharmacology Published Online August 2014. DOI 10.1007/s00280-014-2549-y
      Species: Human
      Sample Type: Cell Lysate

    9. The Presence of HIV-1 Tat Second Exon Delays Fas-Mediated Apoptosis in CD4+ T lymphocytes: a Potential Mechanism for Persistent Viral Production
      Species: Human
      Sample Type: Cell Lysate

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