nCounter® miRNA Expression Assays have been carefully curated to ensure the relevant content and appropriate controls to achieve excellent research results. NanoString utilizes proprietary metrics such as observed read ratios and expression analytics to screen potential content before inclusion in the panel. These methods help ensure that miRNA panel content is heavily weighted toward biologically significant miRNAs. In addition to this data-driven selection process, NanoString also performs literature reviews to ensure that the most relevant miRNAs are included in our panels.
Targeted Discovery for miRNA
nCounter® miRNA Expression Assays have been carefully curated to ensure the relevant content and appropriate controls to achieve excellent research results. NanoString utilizes proprietary metrics such as observed read ratios and expression analytics to screen potential content before inclusion in the panel. These methods help ensure that miRNA panel content is heavily weighted toward biologically significant miRNAs. In addition to this data-driven selection process, NanoString also performs literature reviews to ensure that the most relevant miRNAs are included in our panels.
Controls Ensure Robust Performance
Each nCounter® miRNA panel contains a large number of control probes to ensure robust performance. For the newest panel versions, Human v3 miRNA, Mouse v1.5 miRNA, and Rat v1.5 miRNA, NanoString's miRNA panel controls have been updated to include probes for both hybridization and ligation based on sequences generated by the ERCC (External RNA Controls Consortium).
miRNA Profiling with Excellent Accuracy
nCounter miRNA Expression Assays enable highly multiplexed, direct digital detection and counting of miRNAs in a single reaction without amplification. NanoString offers convenient, fixed-content panels for analysis of miRNA transcriptomes of Human, Mouse, and Rat. nCounter's unbiased and simple workflow enables these panels to generate miRNA expression profiles with great sensitivity and reproducibility. Custom offerings are also available enabling the analysis of focused sub-sets of miRNAs (nCounter Custom à la carte miRNA CodeSets).
See how miRNA fits into our workflow
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| Highly sensitive and specific quantification of miRNA in serum and plasma samples. | Automated past-hybridization processing on nCounter systems. | nSolver utilizes a variety of appropriate normalization methodologies in all samples to provide accurate analysis. |
Precision & Accuracy
No amplification required
High specificity and sensitivity
Sample Compatibility
Total purified RNA
FFPE-derived total RNA
Customizable
Standard - miRBase coverage panel sets
Custom à la carte sets
